PC Linker (photocleavable)
Photocleavable Linker (PC Linker) often used in the design of multi-functional single-stranded nucleotide conjugates for the selection of novel DNA or RNA-based catalysts in vitro. Photocleavable PC linker modified oligonucleotide are demonstrated in Bruker Daltonik's genoSNIP, a MALDI-TOF MS based assay system for SNP detection.
Photocleavable PC linker contained a non-nucleosidic group that can be used to link two nucleotide sequences through a short, UV photo-cleavable C3 spacer arm. Upon irradiation by UV light, photo-cleavage released oligonucleotide contain a 5-phosphorylated group with greater hybridization affinity for a complementary DNA strand than PC spacer.
Photocleavable PC linker can be incorporate at any position within an oligonucleotide. Contact Bio-Synthesis for service details.
XX-15A uv lamp 365nm 台式紫外线灯
Cleavage Protocol：
Cleavage occurs by irradiation with near-UV light (300-350 nm, complete cleavage occurs within 5 minutes. Try using a XX-15A UV lamp at a distance of 15 cm (emission peak 365 nm, 300 nm cut-off, 1.1 mW intensity at~31 cm).
上海峰志仪器有限公司现货供应用于激活DNA上切割位的XX-15A 365nm紫外线灯,可选配台式支架和遮光罩。
产品介绍请浏览：美国路阳 XX-15A实验室用紫外线灯
紫外线灯用于基因编辑
研究发现crRNA-c对核酸内切酶降解具有极端稳定性，却会在辐照度为20 mW/cm2的365nm UV光照射下，在30秒内释放出线性crRNA，这为响应性CRISPR编辑提供了一个极好的响应窗口。通过更进一步的研究发现，这种技术应用于CRISPR-Cpf1系统也是可行的，这意味着他们的光激活环状gRNA策略是调控RNA-引导的CRISPR编辑的通用方法。
